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Titel
Glycophenotyping of osteoarthritic cartilage and chondrocytes by RT-qPCR, mass spectrometry, histochemistry with plant/human lectins and lectin localization with a glycoprotein
VerfasserToegel, Stefan ; Bieder, Daniela ; André, Sabine ; Altmann, Friedrich ; Walzer, Sonja M. ; Kaltner, Herbert ; Hofstaetter, Jochen G. ; Windhager, Reinhard ; Gabius, Hans-Joachim
Erschienen in
Arthritis Research & Therapy, 2013, Jg. 15, R147
ErschienenBioMed Central (BMC), 2013
SpracheEnglisch
DokumenttypAufsatz in einer Zeitschrift
ISSN1478-6362
URNurn:nbn:at:at-ubbw:3-601 Persistent Identifier (URN)
DOIdoi:10.1186/ar4330 
Zugriffsbeschränkung
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Glycophenotyping of osteoarthritic cartilage and chondrocytes by RT-qPCR, mass spectrometry, histochemistry with plant/human lectins and lectin localization with a glycoprotein [1.27 mb]
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Zusammenfassung (Englisch)

Introduction:

This study aimed to characterize the glycophenotype of osteoarthritic cartilage and human chondrocytes.

Methods:

Articular knee cartilage was obtained from nine osteoarthritis (OA) patients. mRNA levels for 27 glycosyltransferases were analyzed in OA chondrocytes using RT-qPCR. Additionally, N- and O-glycans were quantified using mass-spectrometry. Histologically, two cartilage areas with Mankin scores (MS) either 4 or 9 were selected from each patient representing areas of mild and severe OA, respectively. Tissue sections were stained with (1) a selected panel of plant lectins for probing into the OA glycophenotype, (2) the human lectins galectins-1 and -3, and (3) the glycoprotein asialofetuin (ASF) for visualizing -galactoside-specific endogenous lectins.

Results:

We found that OA chondrocytes expressed oligomannosidic structures as well as non-, mono- and disialylated complex-type N-glycans, and core 2 O-glycans. Reflecting B4GALNT3 mRNA presence in OA chondrocytes, LacdiNAc-terminated structures were detected. Staining profiles for plant and human lectins were dependent on the grade of cartilage degeneration, and ASF-positive cells were observed in significantly higher rates in areas of severe degeneration.

Conclusions:

In summary, distinct aspects of the glycome in OA cartilage are altered with progressing degeneration. In particular, the alterations measured by galectin-3 and the pan-galectin sensor ASF encourage detailed studies of galectin functionality in OA.