Zur Seitenansicht
 

Titelaufnahme

Titel
Suppressed recombination and unique candidate genes in the divergent haplotype encoding Fhb1, a major Fusarium head blight resistance locus in wheat
VerfasserSchweiger, W. ; Steiner, B. ; Vautrin, S. ; Nussbaumer, T. ; Siegwart, G. ; Zamini, M. ; Jungreithmeier, F. ; Gratl, V. ; Lemmens, M. ; Mayer, K.F.X. ; Bérgès, H. ; Adam, G. ; Buerstmayr, H.
Erschienen in
Theoretical and Applied Genetics, Berlin, 2016, Jg. 129, H. 8, S. 1607-1623
ErschienenSpringer, 2016
SpracheEnglisch
DokumenttypAufsatz in einer Zeitschrift
ISSN1432-2242
URNurn:nbn:at:at-ubbw:3-1687 Persistent Identifier (URN)
DOI10.1007/s00122-016-2727-x 
Zugriffsbeschränkung
 Das Werk ist frei verfügbar
Dateien
Suppressed recombination and unique candidate genes in the divergent haplotype encoding Fhb1, a major Fusarium head blight resistance locus in wheat [0.77 mb]
Links
Nachweis
Klassifikation
Zusammenfassung (Englisch)

Fhb1 is a prominent Fusarium head blight resistance locus of wheat, which has been successfully introgressed in adapted breeding material, where it confers a significant increase in overall resistance to the causal pathogen Fusarium graminearum and the fungal virulence factor and mycotoxin deoxynivalenol. The Fhb1 region has been resolved for the susceptible wheat reference genotype Chinese Spring, yet the causal gene itself has not been identified in resistant cultivars. Here, we report the establishment of a 1 Mb contig embracing Fhb1 in the donor line CM-82036. Sequencing revealed that the region of Fhb1 deviates from the Chinese Spring reference in DNA size and gene content, which explains the repressed recombination at the locus in the performed fine mapping. Differences in genes expression between near-isogenic lines segregating for Fhb1 challenged with F. graminearum or treated with mock were investigated in a time-course experiment by RNA sequencing. Several candidate genes were identified, including a pathogen-responsive GDSL lipase absent in susceptible lines. The sequence of the Fhb1 region, the resulting list of candidate genes, and near-diagnostic KASP markers for Fhb1 constitute a valuable resource for breeding and further studies aiming to identify the gene(s) responsible for F. graminearum and deoxynivalenol resistance.

Statistik
Das PDF-Dokument wurde 28 mal heruntergeladen.