Summary The habitat of wild animals is strongly influenced by cultivation and building density of humans. Animals react differently to changes of their habitat. This study investigates the reproductive output of Great tits (Parus major) in the Austrian capital city Vienna. The study compares the number of eggs and the chick mass in 129 nests in nesting boxes between four different types of habitat. The habitat types were defined as forest, agricultural area, suburban area and inner city. 19 characteristics of the nesting habitat (parameters of the habitat) were collected as well. Uni- and multivariate regression models linked habitat parameters to the reproductive output. There were significant more eggs found in the forest compared to the inner city. The chick mass was significant higher in the forest than in the suburban area and than in the inner city. Also in agricultural areas, the chick mass was significant higher compared to the inner city. In summary, the mean chick mass was highest in the forest, second in agricultural areas, third in suburban areas and lowest in the inner city. In linear regression models, deciduous trees, dead wood, the presence of oaks, herbaceous plants and areas without vegetation showed a positive influence on chick mass. In opposite, concrete, the presence of buildings, big conifers and meadows decreased chick mass. Oaks, herbaceous plants showed a positive impact on chick mass, whereas buildings and big conifers influenced the chick mass negatively in a multivariate regression model. The negative impact of big conifers is caused by the fact that big conifers did not occur in the forest with the highest reproductive output. This work shows that the type of habitat and habitat parameters influence the chick mass of Great tits in Vienna.