The project Regiokorn stands for cereals at the moment rye (Secale cereale) and spelt wheat (Triticum spelta) - grown, processed and marketed in South Tyrol. In the future, bread wheat (T. aestivum) should be included in the project in order to broaden the range of baked goods. Adequate supply with nitrogen is crucial for the baking quality of bread wheat. Since within the Regiokorn project no soluble mineral fertilizers are used, the effects of fertilization with biogas manure on baking quality were investigated. Therefore, field trials were carried out considering two harvest years (2014, 2015), three wheat varieties (Capo, Pireneo, Wiwa), two precrops (potato, silage maize), two fertilization managements (splitting, no splitting), and four fertilization levels (0%, 75%, 100% and 125% of the target level). Protein content, protein yield, falling number, sedimentation value, mixing properties and dough extension properties were investigated. The statistical analysis showed positive effects of fertilization with biogas manure on protein content and protein yield. There were significant differences between varieties and harvest years within all quality traits. If adequate varieties and precrops are chosen, a fertilization with biogas manure leads to suitable baking qualities, as long as weather conditions are not extraordinary unfavorable. For definitive predictions, however, baking tests are recommended.