In the context of the „EU-Life-Project Untere Marchauen“ six Konik mares were settled in an enclosure within the Riparian nature reserve „Marchegg“. Following their arrival within this new surrounding and given the high amount of mosquitoes prevailing, which was anticipated given the nature of this Riparian reserve, the objectives of this study were to analyse how the horses would adapt to their habitat within a 24-hour frame and how they would cope with the mosquitoes. The sampling and the mosquito trapping were carried out during three time pe-riods throughout a day over a period of 22 days, in summer 2015. In general, open areas were preferred. Pastures were mainly used for grazing and resting, preferably during the nights. The woods were mainly used in the morning and in general utilized for standing, grazing and moving. In the afternoon, when the temperatures were elevated, the horses preferred being on pasture, on the levee and under the canopy of trees. This indicates that they prove to be the best places for horses when dealing with heat and insects, as assumed. Behaviours assumed to be related to insect avoidance were shown mostly in the afternoon, whereas mosquitoes were most abundant in the evening. Thus, no positive statistical relationship between comfort behaviour and the amount of mosquitoes could be established. The amount of individuals in this study was low, mostly on the account of dry and hot weather conditions in 2015. Based on the results of this study, it is therefore not possible to establish a general relationship between mosquitoes and insect avoidance behaviour of the horses or their habitat use. Further analyses need to be carried out in order to get more valid results. It can be concluded that the peaks of comfort movements in the afternoon originated from other insects, such as tabanids, which tend to be active in the afternoons during high temperatures.