Following the documented failure of conventional batch extraction techniques to measure nutrient availability across different soil types and climate regimes, alternative techniques such as diffusive gradients in thin-films (DGT) have been proposed as alternatives. The objective of this study was to characterize the potential of DGT as a tool to assess nutrient availability, and to investigate the capacity of DGT to predict crop response to P and micronutrient concentrations. 119 soil samples were taken from long-term field experiments in four climate/soil zones throughout Austria, in attempts to represent the dominant agricultural landscapes in the country. P and micronutrient (Fe, Mn, Cu) concentrations were measured with DGT and conventional extraction protocols (EDTA, CAL). Subsequently, DGT was compared to extraction techniques in order to (1) determine the influence of soil edaphic (pH, CaCO3) properties on DGT measurements and to (2) determine the capability of DGT to predict crop response to varying P and micronutrient concentrations under field conditions. Our results indicate that DGT is less influenced by CaCO3 and pH fluctuations, while extraction techniques were highly dependent on these soil physicochemical properties. Though DGT P concentrations were moderately correlated (R2=0.50) with CAL values across all 4 sites, the relationship was strengthened after separating soil test values based on carbonate content (R2=0.76, R2=0.85). DGT concentrations exhibited saturating non-linear behavior in relation to relative yield of wheat (Triticum aestivum) and barley (Hordeum vulgare); although this relationship was statistically insignificant it was indicative of typical dose-response relationships which are commonly reported for macro and micronutrients. Interestingly, DGT Mn concentrations exhibited similar behavior, which highlight the control of soil edaphic properties on micronutrient availability and the relative importance of micronutrient nutrition in our study system.