This master thesis deals with the current precarious traffic situation for cyclists between the University of Natural Resources and Life Sciencess main locations “Türkenschanze” and “Muthgasse”. Three suitable routes between campus “Türkenschanze” and “Muthgasse” were drafted (route A, B and C) and systematically tested for their suitability by an assessment analysis. To collect the required data for this assessment, inspection trips by bicycle were made. In the course of the assessment process, existing deficiencies were identified and the actual conditions were compared to ideal requirements, which are based on the official Austrian bicycle traffic guidelines (RVS 03.02.13 Radverkehr). Junctions and street segments were individually analyzed using different assessment indicators. The evaluation results show that route A, which leads via railway station Spittelau and Donaukanalradweg, offers the most bicycle-friendly streets but many bad connected junctions too. Route C has the worst results, mainly because of the routes length. The alignment via railway station Heiligenstadt is probably a too long way round. The most suitable variant is Route B which achieved 72% of the possible goals. The route-specific advantages are the shortness and directness for cyclists. Unfortunately more than half of the route is in need of improvement. Provided recommendations for improvements are for example in the region of Türkenschanze, a multi-purpose lane “Mehrzweckstreifen” on Peter-Jordan-Straßes uphill side in the section Dänenstraße Linnéplatz, or a conversion of the section Linnéplatz - Billrothstraße to a one-way street with a contraflow bicycle lane “Radfahrstreifen” in uphill direction. In the surrounding area of universitys location Muthgasse, a bicycle ramp at the north side of Heiligenstädter Brücke is proposed as well as a bidirectional bike path “Zweirichtungsradweg” in Muthgasse.