Methane oxidising bacteria (methanotrophs) are ubiquitous,acting as bio-filters for greenhouse gas methane in the environments.Hence, methanotrophs play a central role in eco-processes affecting climate change (e.g. global warming).Even though methanotrophs were intensively studied in the last decades,little is known about the feasibility of comparing the experimental results across the different laboratories performing the same procedures.Similarly,little is known about how methanotroph function and community composition are influenced by the overall microbial diversities; their resistance and resilience against disturbances regarding their community structure, abundance and function.In this study, the impacts of inter- and intra-laboratory variations of environmental DNA extraction were evaluated by methanotroph community analysis.Methanotrophs were applied as a model system in a mesocosm study.Different total microbial and methanotroph community assemblies were applied as starting materials and the dynamics of the methanotroph and bacterial community compositions as well as methane oxidation as function were followed over a period.The results showed that methanotrophs recolonized from the upper innoculum layers. Surprisingly, different microbial assembly histories did not affect methanotroph community diversity,while did significantly influence total microbial diversity.The recovery and succession of methanotroph communities followed a similar pattern between different microbial assemblies.Flood and drought as disturbances were applied to the same mesocosms to assess the response and resilience of methanotroph community composition and function to and against these perturbations. Methanotroph compositions showed remarkable resistance to both disturbances even though they were embedded in different microbial communities. Methanotroph abundance and function (methane oxidation potentials) indicated high level resilience against the varying of moisture content.