The aim of this study was the evaluation of the eco-physiological responses of tomato genotypes from different breeding environments to drought stress. In total, thirteen genotypes of different origins were screened. They were categorized in old and new cultivars, conventionally and organically bred ones, and genotypes bred in different continents, as well as parental lines and progenies. These genotypes were a subset of genotypes tested within the framework of the breeding programme “Organic Outdoor Tomato Project” in field experiments at the University of Göttingen. A pot experiment with two levels of water supply (normal, deficient) was conducted. After growing the plants in pots for five weeks, chlorophyll fluorescence and chlorophyll concentration of leaves were measured and current water content and osmotic potential of single leaves were assessed. After eight weeks in the pots, fresh weight, dry weight, and current water content of shoots and roots were determined. Results show lower fresh weight of roots and shoots (shoot + leaves) of stressed plants. Dry weight of shoots of ‘Matina did not show a significant difference, whereas ‘Clou, ‘Golden currant, ‘Cerise gelb and ‘Zuckertraube showed just a low significant difference. All the remaining genotypes did not show a significant difference. Root dry weight differed significantly in ‘Phantasia, ‘NC-37, ‘226-11-4 and ‘Cerise gelb. Now and low significant differences respectively were found in ‘Philovita, ‘Hildares, ‘Matina, ‘LBR-11, ‘Primavera, ‘Clou, ‘Resi, ‘Golden currant und ‘Zuckertraube. Physiological parameters did show differences between non-stressed and stressed plants but they do not provide conclusive information about evaluation of drought stress tolerance.