Soil hydraulic properties are subject to a high natural spatial variability. This makes the estimation of representative parameters, e.g. for modeling soil water balances, highly challenging. Therefore, the main objective was to compare presented new sampling approach to a standard one by capturing changes of the hydraulic properties within the depth and distinction of it regarding different soil horizons for two contrasting types of land use. To derive the unsaturated hydraulic conductivity, two contrasting land uses and soil types were analyzed: A Chernozem at the agricultural research farm in Gross-Enzersdorf and a Luvisol at the forest demonstration center in the Rosalia mountains (both Lower Austria) were sampled in steps of 5 cm down a vertical transect. The samples were measured using the evaporation method (HYPROP device, UMS GmbH Germany). The evaporation data was used to subsequently derive the hydraulic conductivity and retention functions using parameter fitting procedures. The simulation software HYDRUS was used to assess the impact of different soil hydraulic properties regarding depth, as well as, varying soil horizons on water balance components. The simulation results showed how water movement and storage in the soil is affected by the soil hydraulic parameters and climatic conditions and proved that the proposed sampling method better reflects soil profiles hydraulic properties than the standard approach.