Wheat is one of the most important crops. However, the productivity is often limited by the occurrence of pest organisms. Fusarium head blight (FHB), which is mainly caused by Fusarium graminearum, is one of the most devastating diseases affecting wheat and other gramineous crop species. Besides negative effects concerning yield and seed quality, the production of mycotoxins poses a hazard to human and animal health. Therefore, the development of resistant cultivars is a declared goal for many breeders and plant scientists. The genetic resistance mechanisms are still largely unknown, despite various studies and intensive breeding strategies against FHB. Numerous quantitative trait loci (QTL) have been identified to be associated with the resistance trait, but none of the underlying genes has been cloned yet. One major QTL, namely Fhb1 on chromosome 3B, was found to be most promising and has thus been used for marker-assisted breeding. The aim of the master thesis at hand was the selection of near-isogenic lines (NILs) with recombinations in the Fhb1 region for the fine mapping of the QTL and finally to support gene cloning. Initially, 2080 NILs in the F2 generation for the Fhb1 region were screened using two molecular markers (XGWM493 and XBARC133) flanking the Fhb1 QTL. Thus, 121 lines with recombinations between the two markers were identified with most of the lines heterozygous for one of the markers. In a second selection step in the F3 generation, 85 NILs were identified to be homozygous and recombinant in the Fhb1 region. The putative Fhb1 region of the recombinant lines was further characterized using two additional markers, UMN10 and Snp3BS-8, allowing the separation of the lines in five haplotypes. These lines serve as an essential genetic resource for subsequent fine mapping, in order to narrow down this large QTL region and to facilitate cloning of the Fhb1 resistance gene(s).