Abstract Heterodera schachtii Schmidt, has been considered for more than a century the most important nematode pest of sugar beet. In plantnematode interactions, plant defense responses to parasitic nematodes have the potential to become part of management strategies to increase agricultural productivity. The volatile MeJA was known to activate systemic response of plants attacked by harmful pathogens. At certain external stimuli (eg. wounding), the jasmonate biosynthesis is activated leading to gene expression changes and hormone accumulation. The aim of the current study was to elucidate the role of JA for the Arabidopsis thaliana Heterodera schachtii interaction. The results revealed that MeJA or SHAM shoot application had no effect on nematode attraction. However, MeJA treatment significantly lowered the infection rate at 24h and 48h after J2 inoculation, as well as, significantly reduced female development, while SHAM shoot application was found to have no significant effects. The quantitative expression analysis on JA-related genes showed a significant down-regulation of PDF1.2A in the roots but was up-regulated in the shoots after 24h from J2 inoculation without hormone application. Hormone application showed different gene expression in roots, COI1 and JAR1 were down-regulated, whereas, PDF1.2A was up-regulated. The results of this work clearly show that JA is involved in the A. thaliana H. schachtii interaction and imply that elevated JA levels causes unfavorable condition nematode root infection and development.