Fusarium Head Blight (FHB) is a serious disease of small grain crops such as wheat and barley. FHB is mainly due to infections by the fungi Fusarium graminearum (teleomorph: Gibberella zeae) and F. culmorum. Apart from causing severe yield losses, these fungi can also produce mycotoxins such as deoxynivalenol and zearalenone, preventing contaminated crops to be further used as food or feed. Control of FHB can be achieved through cultural practices (tillage, crop rotation), use of fungicides, resistant cultivars or biological control agents (BCAs). In the present work, three wheat cultivars were inoculated with G. zeae using the kernel spawn method to infect wheat ears in a natural way. Three different BCAs, each of which acts through a distinct control mechanism (SAR-inducing, antibiosis, competition for resources) were then sprayed onto the wheat plants at different developmental stages and in different combinations. To find out which BCA combination acted best and to which extent FHB symptoms were reduced, the disease incidence DI (%), disease severity DS (%) and percentage of Fusarium damaged kernels FDK were scored and the disease intensity DINT (%) computed. Wheat cultivars exerted the strongest effects on disease development. Neither of the BCA treatments nor the fungicide Folicur® were able to reduce DI (%) or FDK. When applied alone, BCA P183 reduced the area under the disease pressure curve (AUDPC) DS (%) by 25% (P<0.05) compared to the control, similar to the fungicide (30% reduction, P<0.05). BCA combinations also decreased the AUDPC DS (%). Analysis of the AUDPC DINT (%) showed similar results. There is some evidence that cultivars responded differently to the BCA treatments. As even the fungicide did not control FHB to a high degree, it is assumed that more conclusive results can be obtained under less pronounced disease pressure, which seems to have been exceptionally high.