HEV was identified for the first time as the main cause of epidemic and endemic enteral diseases in 1980 in India. Four major genotypes (1-4) occur in mammals and one HEV genotype is specific for bird infections. An epidemic spread of HEV by contaminated water is typical for areas with poor sanitation. This makes HEV to a major health problem in the developing countries. In industrialized countries, the risk of viral infection is related to two main causes: while Genotypes 1 and 2 occur only in human, Genotypes 3 and 4 affect humans and animals (e.g. wild boar and deer). Due to improper preparation of relevant food prior to consumption, a zoonotic spread of Genotypes 3 and 4 is thus a potential risk source of infection exposure. Another possible route of a viral transmission is from human to human through HEV-contaminated blood transfusions (blood, blood plasma). This paper focuses on the development of a Nucleic Acid Amplification Technique (NAT) that can be used to test plasma pool samples for HEV contamination. In the first part of the paper, an analytical method was developed based on a reference material, enabling the detection of low viral loads of 10^1 International Units per ML (IU/ML) in samples. In order to fulfill this task, it was possible to optimize the isolation of viral genome, the reverse transcription (RT), the polymerase chain reaction (PCR) and agarose gel electrophoresis. The second part of this paper deals with the standardized procedural testing of plasma pool samples. A wide range of plasma pool samples was available when selecting samples. Samples were submitted by various companies to the Austrian Agency for Health and Food Safety (Österreichischen Agentur für Gesundheit und Ernährungssicherheit GmbH), Medical Market Supervision (Medizinmarktaufsicht), for licensing. From among the total of 16 tested samples, one sample tested positive for HEV contamination.