The aim of this thesis is to evaluate the overall economical effects (costs/benefits) of two means of transport bicycle and car. To do this, several indicators of the three dimensions of sustainability are analysed: health benefit, costs for operation, travel time, infrastructure, noise, accidents, air pollution and CO2 (for global warming potential). To be able to compare the different indicators involved, the effects of both means of transport are put into a framework using -cent as the unit of indicators per cycled or driven kilometre. Observing the aggregated indicators, the external benefit per cycled kilometre is 81,47 ct/km and the overall costs per cycled kilometre are 1,55 ct/km. Concerning the car, external costs per driven kilometre of 4,35 ct/km are generated and overall costs per driven kilometre are 98,38 ct/km. In a next step, these indicators are applied and calculated on traffic scenarios in Vienna, taking into account the number of car trips which actually could be substituted by bicycle. Based on the actual modal split of Vienna (4 % of all trips by bicycle, 25 % by car drivers), two scenarios are calculated. Starting from status quo, the external benefit of the bicycle outbalances external costs of the car by 35 mio. /year. Regarding scenario 1 (6 % bicycle, 23 % car drivers), an additional benefit of 111 mio. /year can be achieved compared to the status quo. Scenario 2 (7 % bicycle, 22 % car drivers) shows an additional benefit of 167 mio. /year compared to the status quo.