Fusarium head blight (FHB) is caused by Fusarium graminearum and is one of the most serious plant diseases in wheat producing areas over the world. In addition to yield losses, the pathogen is producing deoxynivalenol (DON), which has devastating effects on the nutrition of humans and animals. There are several ways to control the pathogen: cultural control, the breeding of resistant wheat lines and the promising possibility for the use of microbial antagonists for biological control of the pathogen. The main mechanisms of potential antagonists against the pathogen are the excretion of antibiotics and competition effects. The overall aim of this masterwork was to examine eleven potential “biological control agents” (BCAs) for their ability to inhibit the effect of F. graminearum under field conditions and to rank them according to their effectiveness. Four bacterial and six fungal strains, including the biopesticide Botector, cow milk, the fungicide Folicur and a control treatment with water, were tested on the cultivars “Capo”, “Kronjet” and “Trappe”. All of the BCAs significantly reduced the symptoms of FHB in previously made glasshouse trials. Based on the collected data and the effectiveness of the antagonists, a statistical ranking of the treatments was made. All potential BCAs had advantageous effects on the parameters, except the treatments IFA 134 and B020SP, which increased the percentage of fusarium damaged kernels (FDKProz) on two wheat genotypes. The most efficient treatments in decreasing the level of the disease parameters were the fungal antagonists Botector, P183 and P169. They decreased the disease severity (DS) with -57,8%, -54,5% and 53,6%. The treatment Botector reached nearly 70% of the efficacy of Folicur, in reducing DS. P183 (-66,9%) and Botector (-62,9%) did not statistically differ from the fungicide (-77,9%) in their ability to reduce FDKProz.